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KMID : 0352519840210010177
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Korea Univercity Medical Journal
1984 Volume.21 No. 1 p.177 ~ p.184
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Comparative study on the indirect immunofluorescent antibody test, complement fixation test and ELISA in diagnosis of human clonorchiasis
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咸姃希/Hahm, Jeong-Hee
李駿商/林漢鍾/Lee, Joon-Sang/Rim, Han-Jong
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Abstract
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Human clonorchiasis is one of the most common trematode diseases and of very important public health problem in Korea. A number of serological techniques have been applied to the diagnosis of parasitic diseases, and efforts in recent years have been made for the development of new promising serological techniques.
In this study the sensitivity and the specificity of the indirect immunofluorescent antibody test(IFAT), the complement fixation test(CFT) and the enzyme-linked immunosorbent assay (ELISA) were investigated with 55 human clonorchiasis sera, 16 sera of other parasitic infections and 9 sera of non-infected human cases.
The IFAT was performed using frozen sectioned antigen of adult Clonorchis sinensis, the
CFT was performed with ether extracted antigen prepared from adult C.sinensis.
The ELISA was performed with Clonorchis veronal buffered saline antigen, peroxidase
conjugated antihuman Ig G and ortho-phenylenediamine as a substrate by micro-method. The results obtained were as follows:
1. The positive reactions in 34 (61.80o) out of 55 clonorchiasis sera at a dilution of 1116 or below and in one (4.0%) out of 25 control sera were detected by the IFAT.
2. The positive reactions in 31 (56.400) out of 55 clonorchiasis sera at a dilution of 1/8 or below and in 3 (12.00%) out of 25 control sera were detected by the CFT.
3. Fourty-three (78.2%) out of 55 clonorchiasis sera were positive at absorbance 1.000 or below by the ELISA. However, 25 control sera tested by the ELISA were negative.
4. Among the 43 Clonorchis positive sera detected by the ELISA, positive reactions were observed in 300 (76.700) sera in the IFAT and 30 (69.8%5) sera in the CFT. Among the 12 Clonorchis negative sera tested by the ELISA, one gave positive reaction by the IFAT
and the CFT.
5. When the results were compared between the IFAT and the CFT using 55 human clonorchiasis sera showed that 50 (90.90) out of the 55 clonorchiasis sera gave same reactions, and the titers of two immunological techniques were almost-corresponding.
These findings indicated that the ELISA was the most sensitive and specific in the diagnosis of human clonorchiasis among three serological tests.
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